Single-molecule sequence detection via microfluidic planar extensional flow at a stagnation point
نویسندگان
چکیده
منابع مشابه
Single-molecule sequence detection via microfluidic planar extensional flow at a stagnation point.
We demonstrate the use of a microfluidic stagnation point flow to trap and extend single molecules of double-stranded (ds) genomic DNA for detection of target sequences along the DNA backbone. Mutant EcoRI-based fluorescent markers are bound sequence-specifically to fluorescently labeled ds lambda-DNA. The marker-DNA complexes are introduced into a microfluidic cross slot consisting of flow cha...
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The classical Hiemenz solution describes incompressible two-dimensional stagnation point flow at a solid wall. We consider an unsteady version of this problem, examining particularly the response close to the wall when the solution at infinity is modulated in time by a periodic factor of specified amplitude and frequency. While this problem has already been tackled in the literature for general...
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We demonstrate the feasibility of a single-molecule microfluidic approach to both sequence detection and obtaining kinetic information for restriction endonucleases on dsDNA. In this method, a microfluidic stagnation point flow is designed to trap, hold, and linearize double-stranded (ds) genomic DNA to which a restriction endonuclease has been pre-bound sequence-specifically. By introducing th...
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Kühnemund, M. 2016. Single Molecule Detection. Microfluidic Automation and Digital Quantification. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1189. 57 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9498-8. Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, ...
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ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2010
ISSN: 1473-0197,1473-0189
DOI: 10.1039/b926847b